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rat cd146 apc  (Miltenyi Biotec)


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    Miltenyi Biotec rat cd146 apc
    Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, <t>CD146</t> (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Rat Cd146 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat cd146 apc/product/Miltenyi Biotec
    Average 94 stars, based on 5 article reviews
    rat cd146 apc - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis"

    Article Title: Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.04.015

    Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, CD146 (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, CD146 (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing, Marker, Control, Staining, Quantitative Proteomics



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    Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, <t>CD146</t> (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, <t>CD146</t> (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, CD146 (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis

    doi: 10.1016/j.jare.2025.04.015

    Figure Lengend Snippet: Donor PC therapy restores CMA in host PCs in damaged tissue. (A) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (green) with a PC marker, α-SMA (red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas (injured) and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. Right panels show bigger magnification of double positive cells. ( B ) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, PDGFRβ (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with control adMSCs and with donor brain WT or KO PCs. Right panels show bigger magnification of double positive cells. Nuclei were stained with DAPI (blue). Scale bars: 100 µm. (C) Representative images showing co-localization of puncta pattern expression of LAMP-2A protein (in green) with a PC marker, CD146 (in red), analyzed in host PCs of microvessels (v) of control mice, in mice brain with lesioned areas and treated intravenously with donor brain PC. Right panels show bigger magnification of double positive cells (Scale bars: 20 µm). Nuclei were stained with DAPI (blue). Scale bars: 100 µm. ( D ) Relative quantification of percentage of PCs in brain microvessels expressing the PC marker α -SMA (n = 40 α -SMA + PCs per condition), PDGFRβ (n = 20 PDGFRβ + PCs per condition), or CD146 (n = 35 CD146 + PCs per condition) and the CMA receptor LAMP-2A (PC/LAMP-2A +/+ ) over total PCs. All data represent mean ± SD obtained from at least, three experiments represented as dots, independently; * p < 0.05; ** p < 0.01; *** p < 0.001; ns indicates no statistical significance (ANOVA with Tukey’s post-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: For fluorescent double labelling, mouse anti-alpha-smooth muscle actin (α-SMA; Abcam, ab7817), goat anti-platelet derived growth factor receptor beta (PDGFRβ; R&D Systems, BAF1042), or rat CD146-APC (Miltenyi Biotec, 130–118-408) antibodies were used in combination with rabbit anti-LAMP-2A (Invitrogen, 51–2200) antibody.

    Techniques: Expressing, Marker, Control, Staining, Quantitative Proteomics